human fibronectin duoset elisa Search Results


human fibronectin  (R&D Systems)
94
R&D Systems human fibronectin
( A-B ) Graphs show <t>fibronectin</t> (μg/ml) and hyaluronic acid (ng/ml) levels in the media of bioreactor-cultured hPCLS after 24 rest and then 24h or 72h culture ± fib stim (TGFβ1/PDGFββ) ± Alk5i, numbers on bars show fold change compared to 48h control. ( C ) Graphs show mRNA levels of collagen 1A1, αSMA and TIMP1 in hPCLS at t=0 and after 24h rest plus 72h culture ± fib stim ± Alk5i, numbers on bars show fold change compared to t=0. ( D ) Representative 100x magnification images of αSMA (left panel) and Picrosirius red (right panel) stained hPCLS from three different donor livers at t=0, and after 24h rest plus 72h culture (4-days) ± fib stim ± Alk5i. Scale bars equal 200 µm. ( E ) Representative 200x magnification image of Picrosirius red stained hPCLS form donor 4. ( F ) Graph shows the percentage area of picrosirius red stained tissue in hPCLS at t=0, and after 24h rest plus 72h culture ± fib stim ± Alk5i (96h total culture). Data are mean ± SEM in n=4 independent slice experiments. P values were calculated using an Anova with Tukey’s multiple comparisons test (* P <0.05, ** P <0.01, *** P <0.001 and **** P <0.0001).
Human Fibronectin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fibronectin+duoset+elisa/bio_rxiv__331173-68-17-20?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
human fibronectin - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
fibronectin duoset elisa  (R&D Systems)
94
R&D Systems fibronectin duoset elisa
Figure 1. αSMA gene expression and protein levels are reduced in TGFβ1-activated fibroblasts following ketotifen treatment. (A, B) HDFa and (C, D) WS1 fibroblasts were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. Gene expression of αSMA (ACTA2) was measured using RT-qPCR and normalized to housekeeping gene HPRT. HDFa cells were treated for 48 h under the same conditions and probed for αSMA protein by western blot. (E) A representative image of the blots is shown (left) and semi-quantitative assessments plotted (right) using GAPDH as loading control. Full-length blots are available in Supplementary Fig. 4. (F) COL1A1 was measured in HDFa cells treated with conditions using 25 μM ketotifen. Protein levels of pro-collagen 1α1 (G) and <t>fibronectin</t> (H) were determined by <t>ELISA</t> staining kits. Data shown as mean ± SEM. n = 3–6 per treatment condition. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. αSMA alpha-smooth muscle actin, GAPDH glyceraldehyde 3-phosphate dehydrogenase, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TGFβ1 transforming growth factor-beta.
Fibronectin Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fibronectin+duoset+elisa/pm38528089-313-16-20?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
fibronectin duoset elisa - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier

Image Search Results


( A-B ) Graphs show fibronectin (μg/ml) and hyaluronic acid (ng/ml) levels in the media of bioreactor-cultured hPCLS after 24 rest and then 24h or 72h culture ± fib stim (TGFβ1/PDGFββ) ± Alk5i, numbers on bars show fold change compared to 48h control. ( C ) Graphs show mRNA levels of collagen 1A1, αSMA and TIMP1 in hPCLS at t=0 and after 24h rest plus 72h culture ± fib stim ± Alk5i, numbers on bars show fold change compared to t=0. ( D ) Representative 100x magnification images of αSMA (left panel) and Picrosirius red (right panel) stained hPCLS from three different donor livers at t=0, and after 24h rest plus 72h culture (4-days) ± fib stim ± Alk5i. Scale bars equal 200 µm. ( E ) Representative 200x magnification image of Picrosirius red stained hPCLS form donor 4. ( F ) Graph shows the percentage area of picrosirius red stained tissue in hPCLS at t=0, and after 24h rest plus 72h culture ± fib stim ± Alk5i (96h total culture). Data are mean ± SEM in n=4 independent slice experiments. P values were calculated using an Anova with Tukey’s multiple comparisons test (* P <0.05, ** P <0.01, *** P <0.001 and **** P <0.0001).

Journal: bioRxiv

Article Title: A novel bioreactor technology for modelling fibrosis in human and rodent precision-cut liver slices

doi: 10.1101/331173

Figure Lengend Snippet: ( A-B ) Graphs show fibronectin (μg/ml) and hyaluronic acid (ng/ml) levels in the media of bioreactor-cultured hPCLS after 24 rest and then 24h or 72h culture ± fib stim (TGFβ1/PDGFββ) ± Alk5i, numbers on bars show fold change compared to 48h control. ( C ) Graphs show mRNA levels of collagen 1A1, αSMA and TIMP1 in hPCLS at t=0 and after 24h rest plus 72h culture ± fib stim ± Alk5i, numbers on bars show fold change compared to t=0. ( D ) Representative 100x magnification images of αSMA (left panel) and Picrosirius red (right panel) stained hPCLS from three different donor livers at t=0, and after 24h rest plus 72h culture (4-days) ± fib stim ± Alk5i. Scale bars equal 200 µm. ( E ) Representative 200x magnification image of Picrosirius red stained hPCLS form donor 4. ( F ) Graph shows the percentage area of picrosirius red stained tissue in hPCLS at t=0, and after 24h rest plus 72h culture ± fib stim ± Alk5i (96h total culture). Data are mean ± SEM in n=4 independent slice experiments. P values were calculated using an Anova with Tukey’s multiple comparisons test (* P <0.05, ** P <0.01, *** P <0.001 and **** P <0.0001).

Article Snippet: ELISA quantifications for rat COL1a1 (LS-F11152, LSBio, UK), human (E88-129) and rat (E110-125) albumin (Bethyl laboratories, UK), human fibronectin (DY1918-05, R&D systems, UK) and human hyaluronic acid (DY2089, R&D systems, UK) were performed as per manufacturer’s instructions.

Techniques: Cell Culture, Staining

Figure 1. αSMA gene expression and protein levels are reduced in TGFβ1-activated fibroblasts following ketotifen treatment. (A, B) HDFa and (C, D) WS1 fibroblasts were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. Gene expression of αSMA (ACTA2) was measured using RT-qPCR and normalized to housekeeping gene HPRT. HDFa cells were treated for 48 h under the same conditions and probed for αSMA protein by western blot. (E) A representative image of the blots is shown (left) and semi-quantitative assessments plotted (right) using GAPDH as loading control. Full-length blots are available in Supplementary Fig. 4. (F) COL1A1 was measured in HDFa cells treated with conditions using 25 μM ketotifen. Protein levels of pro-collagen 1α1 (G) and fibronectin (H) were determined by ELISA staining kits. Data shown as mean ± SEM. n = 3–6 per treatment condition. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. αSMA alpha-smooth muscle actin, GAPDH glyceraldehyde 3-phosphate dehydrogenase, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TGFβ1 transforming growth factor-beta.

Journal: Scientific reports

Article Title: Ketotifen directly modifies the fibrotic response of human skin fibroblasts.

doi: 10.1038/s41598-024-57776-7

Figure Lengend Snippet: Figure 1. αSMA gene expression and protein levels are reduced in TGFβ1-activated fibroblasts following ketotifen treatment. (A, B) HDFa and (C, D) WS1 fibroblasts were treated for 48 h with DMEM supplemented with 10% FBS as mock, 10 μM or 25 μM ketotifen, 10 ng/mL TGFβ1, or 10 ng/mL TGFβ1 with ketotifen added during the final 24 h. Gene expression of αSMA (ACTA2) was measured using RT-qPCR and normalized to housekeeping gene HPRT. HDFa cells were treated for 48 h under the same conditions and probed for αSMA protein by western blot. (E) A representative image of the blots is shown (left) and semi-quantitative assessments plotted (right) using GAPDH as loading control. Full-length blots are available in Supplementary Fig. 4. (F) COL1A1 was measured in HDFa cells treated with conditions using 25 μM ketotifen. Protein levels of pro-collagen 1α1 (G) and fibronectin (H) were determined by ELISA staining kits. Data shown as mean ± SEM. n = 3–6 per treatment condition. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. αSMA alpha-smooth muscle actin, GAPDH glyceraldehyde 3-phosphate dehydrogenase, HDFa human dermal fibroblasts (adult), SEM standard error of the mean, TGFβ1 transforming growth factor-beta.

Article Snippet: Antibodies used for protein concentration measurements were from pro-collagen 1α1 DuoSet ELISA (DY6220-05, R&D Systems) and fibronectin DuoSet ELISA (DY1918-05, R&D Systems) staining kits.

Techniques: Gene Expression, Quantitative RT-PCR, Western Blot, Control, Enzyme-linked Immunosorbent Assay, Staining